AbstractThe purpose of project is to study the possibility for Borås Energy & Environment to build and run a commercial ethanol production facility in Borås. The project also studies the technology for the production of ethanol using renewable energy, e.g. lignocelluloses with focus on two processes, svag-syra hydrolyse and enzymatic hydrolyse. The technology of the process is based of hydrolysation of biomass (hemicelluloses and cellulose) to sugar and extract it to ethanol. These two techniques will compare with each other to determine which of them that it?s more suitable for ethanol production.

Bioethanol is one alternative energy fuel that can be produced from different types of biomass. Second generation bioethanol comes from fermentation of lignocellulosic material and can be seen as a more environmental friendly alternative as e.g. forest residues and agricultural by-products are used. To be able to use lignocellulosic biomass in ethanol production, it has to be subjected to different treatments first. In this study, aspen sawdust was used as lignocellulosic material.

Ethanol produced from lignocellulosic biomass has the potential to become a promisingalternative to gasoline. In this work the simultaneous saccharification and fermentation (SSF)technology was applied for ethanol production from hardwood with focus on cell growth,ethanol production and contamination.The SSF was performed at PH 5.5 and 35°C for different suspended solid concentrations(8%, 10% and 12%) of pretreated birch slurry which contained 16 % total suspended solids.Two different hexose fermenting yeast strain (Ethanol Red) and pentose fermenting yeaststrain were used.Quantifying the concentration of chemical components and metabolites in the fermentationmedium demonstrated that glucose and xylose are the major fermentable sugars in the slurry.The higher load of slurry (12%) represents a higher content of carbohydrates and potentiallyhigher end concentration of ethanol. Moreover, more lactic acid is produced with the lowerload of slurry (8 % or 10 %), presumably due to a result of a less inhibitory environment forbacterial growth. In this context, acetic acid sticks out as the most important inhibitor withconcentrations of 15.2 and 12.5 and 9.7 g/l respectively in the 12 %, 10 % and 8 % (ofsuspended solids) trials. Using pentose fermenting yeast may lead to higher ethanolproduction, lower xylose uptake and lower lactic acid formation.

One of the most commonly used methods for analysis of polyphenols is the Folin-Ciocalteu (FC) method, where the FC reagent has been reported to interfere with some substances in fruit juices. The interfering substance that is present in the highest amount in most fruit juices is ascorbic acid. In this study, the total phenolic content (TPC) of three fruit juices was analysed by the FC method as well as with an enzymatic method with hydrogen peroxide oxidoreductase type II (HRP) for comparison. To investigate how the interfering ascorbic acid affects the TPC, juice samples were analysed after removal and addition, respectively, of ascorbic acid. The samples were analysed with HPLC both before and after the extraction phase of the FC method.

Catecholamines are stress hormones that are produced and released by a rare tumor called pheochromocytoma. This tumor can cause hypertension which if undiagnosed and untreated leads to death. Since good therapy is available, it is important to find the tumor in time. The most common way to diagnose the tumor is measurement of the biochemical markers; catecholamines and their metabolites, methylated catecholamines.After observation that almost all normetanephrine results for women were higher than the upper reference limit and therefore pathological, the accuracy of the present reference intervals was questioned. Therefore new reference intervals for both urinary catecholamines and methylated catecholamines were developed by analysis of 46 samples using HPLC.

5-hydroxytryptamin (5-HT) is biochemically derived from Tryptophan. Kynurenic acid and quinolinic acid are also known metabolites of tryptophan. 5-HT is considered to play a role in depression and today selective serotonin reuptake inhibitors (SSRIs) are used for treatment of depression. 5-HT may also play a role in pain perception. The aim of this study was to investigate whether a supplement of tryptophan may affect conditions such as depression and/or pain.

Bovine milk is an excellent source of bioactive peptides. The bioactive peptides are in an inactive state in the protein and are released during enzymatic activity, e.g. during cheese ripening. Specific properties of bioactive peptides from ripened cheese has been reported, e.g. antihypertensive, antioxidative, antimicrobial, immunomodulatory and mineral binding.

One of the most serious and economically important defects caused by clostridia in milk products is the late blowing of semi-hard cheeses.Clostridia occur naturally in soil and can contaminate milk through crops contaminated by dung and soil followed by a less successful silage process, that give them opportunity to grow unaerobically. When anaerobic conditions occur, such as storage of semi-hard cheese, they ferment lactic acid to butyric acid and the gases CO2 and H2.At the fusion of Arla and MD Foods, a series of changes were conducted on the MPN method for lactic acid fermentation for clostridia in milk. These changes resulted in an increased accuracy due to an increased number of test tubes and the change of media from MRCM to BBB, Bryant & Burkey Broth, that was thought to be more selective for Cl. tyrobutyricum, the organism mostly found in hard cheese. When the number of dairy farmers that were given quality reduction fines increased, the new method was suspected and a validation was conducted.The validation included inoculation of different clostridia and bacillus strains into BBB substrate and enzymatic testing of positive samples with Rapid ID 32A.

Selenium (Se) is an essential trace element that is involved in several physiological functions in mammals. Mostly, Se is bound to proteins, many of them with enzymatic functions. A group of seleno-depending enzymes, glutathione peroxidases (GSH-Px?s), are important as antioxidants and protects against free radicals. The activity of GSH-Px is strongly regulated by Se storage in the liver.

To enjoy the richness of the grazing cows and high yield during the summers, even during the winter, cheese has been the way to store milk for thousands of years. Nowadays yield is high all year around and the cheese production is possible during all seasons. The aim of this paper is to investi-gate how and why the seasons influence taste and texture of pressed and ripened cheese. Milk is composed of proteins, fat, lactose and water but contains also a lot of vitamins. The quality of milk is very important for the final constitution of cheese.

Ethanol for automotive purposes is an issue much debated and researchers disagree about the benefits of bioethanol. Converting energy-rich crops into ethanol suitable as motor fuel is a complex process and continuous development and improvements of processes and material used are important steps in the work of developing environmental friendly fuels. Grain is usually preserved by drying which consumes a lot of energy. Alternative storage of high-moisture grain in airtight storage systems reduce the energy required to produce bioethanol and studies have also shown that ethanol yields are increased more than 10% in ethanol fermentations of moist grain compared to dry. Airtight storage is often not perfectly airtight so biocontrol agents are necessary in order to preserve the grain.

Milk fat is an important feature in many different milk products and other foodstuffs and it is often crucial for the dairy plants that the milk fat is stable for different manufacturing processes. Lipolysis is the enzymatic degradation of fat and is the one of the causes for an elevated amount of free fatty acids (FFA) in milk. Further, the change in fatty acid (FA) composition in milk can affect the stability of the product and also the manufacturing process. Both internal and external factors, at farm level or at the dairy plants can affect both FA composition and content of FFA. Milking frequency (MF=number of milkings per cow and day) and the composition of feed are two examples of factors generally performed at farm level. The objective of the present study was to evaluate how FA composition of milk and amount of FFA are influenced by two different ingredients supplemented to concentrate.

The quality of bread wheat is a large subject to study. The quality rests on two legs. The first one is the inheritance of specific characters, which can be influenced through plant breeding and type of cultivar. The other one is the environmental influences, such as crop rotation, nutrition, harvest and storage. The latter factors are possibly to affect through cultivation practices, while other factors are not possible to influence on for the grower, such as seasonal changes of weather and the soil content.

Serum thymidine kinase 1 (TK1) activity is used as a tumor marker in disease monitoring in veterinary and human medicine. TK1, an intracellular enzyme, is involved in a salvage pathway of DNA precursor synthesis. TK1 is used in DNA precursor production by catalyzing the transfer of the gamma-phosphate-group from a phosphate-donor to the 5?- hydroxyl-group of thymidine forming thymidine-monophosphate. Nucleoside monophosphosphates are finally converted into thymidine-triphosphates. TK1 activity significantly rises in the G1 and the S phase of the cell cycle.